Real World Drug Discovery: A Chemist's Guide to Biotech and Pharmaceutical Research by Rydzewski Robert M

Author:Rydzewski, Robert M. [Rydzewski, Robert M.]
Language: eng
Format: mobi
Publisher: Elsevier Science
Published: 2008-10-23T16:00:00+00:00

Radiometric techniques bear the distinct disadvantage of generating radioactive waste, which requires special handling and is increasingly expensive to dispose of. Another bead-based method for detecting molecular interactions that doesn’t involve radioisotopes is called AlphaScreen™, which stands for Amplified Luminescent Proximity Homogeneous Assay. This uses two specialized types of bead, a donor and an acceptor. The donor bead, to which one molecule is conjugated, contains a photosensitizer (phthalocyanine) that produces large amounts of singlet oxygen upon laser excitation. Because 1O2 has a very short lifetime, it can diffuse out only about 200 nM, so an acceptor bead, to which the second component is conjugated, will emit light if and only if it’s been drawn close to the donor bead by binding interactions between the two components, as shown in Figure 6.8. This technique has found broad applicability to areas like second messengers, kinases, and protein–protein interactions.[20]

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